Hotspot

atpF-atpH

LSC · 12128-12623 · 496 bp

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Pi 0.0333

MarkerSeek score 79.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 496
variable_sites: 42
pi: 0.0333
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAATAACCCAAGGA	CCAGAGGCGGAAGGAAAAAT	50.0	50.0	57.5	58.2	623-701 bp	1.0	84.4
atpF-atpH_p2	GGATGGCCAATAACCCAAGG	CCAGAGGCGGAAGGAAAAAT	55.0	50.0	58.6	58.2	624-702 bp	1.0	83.3
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CCAGAGGCGGAAGGAAAAAT	50.0	50.0	57.9	58.2	645-723 bp	0.916667	83.2
atpF-atpH_p4	TGGCCAATAACCCAAGGAAA	CCAGAGGCGGAAGGAAAAAT	45.0	50.0	57.3	58.2	621-699 bp	1.0	82.4
atpF-atpH_p5	ATGGCCAATAACCCAAGGAA	CCAGAGGCGGAAGGAAAAAT	45.0	50.0	57.1	58.2	622-700 bp	1.0	80.5