Hotspot

trnS-GCU-trnG-UCC

LSC · 7938-8281 · 344 bp

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Pi 0.0353

MarkerSeek score 75.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 37
pi: 0.0353
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AATCCGACGCTTTAGTCCAC	AGTAATCAAACCGAAAGACCCT	50.0	40.9	58.0	57.4	446-460 bp	1.0	69.0
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	AGTAATCAAACCGAAAGACCCT	50.0	40.9	58.0	57.4	447-461 bp	1.0	68.9
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AATCAAACCGAAAGACCCTT	50.0	40.0	58.0	55.1	444-458 bp	1.0	67.3
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	ATCAAACCGAAAGACCCTTT	50.0	40.0	58.0	55.1	443-457 bp	1.0	67.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AGTAATCAAACCGAAAGACCCT	50.0	40.9	58.2	57.4	501-515 bp	1.0	66.7