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trnK-UUU-rps16

LSC · 4180-4886 · 707 bp

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Pi 0.0501

MarkerSeek score 80.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 102
pi: 0.0501
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	710-998 bp	1.0	91.7
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	CGAGATCTTCGGAAAGTGGG	55.0	55.0	58.0	58.1	792-1080 bp	1.0	91.3
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAAGTGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1472-1592 bp	0.916667	87.4
trnK-UUU-rps16_p4	TCAGGATCAGTCGTGGTCTT	AAAGTGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1468-1588 bp	0.916667	86.9
trnK-UUU-rps16_p5	TTCAGGATCAGTCGTGGTCT	AAAGTGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1469-1589 bp	0.916667	86.9