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trnH-GUG-psbA

LSC · 78-265 · 188 bp

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Pi 0.066

MarkerSeek score 79.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 38
pi: 0.066
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACCGCGCTAATCTTGGTATG	55.0	50.0	58.1	58.1	296-309 bp	1.0	89.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	310-323 bp	1.0	89.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	309-322 bp	1.0	89.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	311-324 bp	1.0	89.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGCGCTAATCTTGGTATGGA	55.0	50.0	58.1	57.9	294-307 bp	1.0	89.8