Hotspot

rps19

IRb · 41542-41820 · 279 bp

Back to Alcantarea

Pi 0

MarkerSeek score 25.60

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCTGACCTCCATACTTAGATCGA	TCTTCCTTTGTTTGATTCACATCT	43.5	33.3	57.7	56.8	429 bp	1.0	40.0
rps19_p2	TGACCTCCATACTTAGATCGAGA	TCTTCCTTTGTTTGATTCACATCT	43.5	33.3	57.7	56.8	427 bp	1.0	40.0
rps19_p3	TCTGACCTCCATACTTAGATCGA	TCTTCCTTTGTTTGATTCACA	43.5	33.3	57.7	53.6	429 bp	1.0	40.0
rps19_p4	TGACCTCCATACTTAGATCGAGA	TCTTCCTTTGTTTGATTCACA	43.5	33.3	57.7	53.6	427 bp	1.0	40.0
rps19_p5	CTGACCTCCATACTTAGATCGAG	TCTTCCTTTGTTTGATTCACATCT	47.8	33.3	57.4	56.8	428 bp	1.0	40.0