Hotspot

rpl16

LSC · 124918-126439 · 1522 bp

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Pi 0.00268

MarkerSeek score 62.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1522
variable_sites: 4
pi: 0.00268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCCAATACCCCTTTTTGT	ACAAACTCTTTCATTCTTTTTCCGT	45.0	32.0	56.6	58.1	1630-1659 bp	1.0	41.6
rpl16_p2	TCCCCAATACCCCTTTTTGT	ACTCTTTCATTCTTTTTCCGT	45.0	33.3	56.6	53.7	1626-1655 bp	1.0	41.6
rpl16_p3	TCCCCAATACCCCTTTTTGTT	ACAAACTCTTTCATTCTTTTTCCGT	42.9	32.0	57.4	58.1	1630-1659 bp	1.0	41.6
rpl16_p4	TCCCCAATACCCCTTTTTGT	ACAAACTCTTTCATTCTTTTTCCG	45.0	33.3	56.6	56.6	1630-1659 bp	1.0	41.6
rpl16_p5	TCCCCAATACCCCTTTTTGT	CAAACTCTTTCATTCTTTTTCCGT	45.0	33.3	56.6	56.6	1629-1658 bp	1.0	41.6