Hotspot

psbC

LSC · 76381-77802 · 1422 bp

Back to Alcantarea

Pi 0

MarkerSeek score 28.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTTCCCAAGAAATCCGTGCA	CCGAGCCAGAACAGAAAAGA	45.0	50.0	58.0	57.8	1582 bp	1.0	78.5
psbC_p2	GCAGCGGAAGATCCTGAATT	CCGAGCCAGAACAGAAAAGA	50.0	50.0	58.3	57.8	1565 bp	1.0	76.0
psbC_p3	AGGTATTCGTGCTTGGATGG	CCGAGCCAGAACAGAAAAGA	50.0	50.0	57.7	57.8	1509 bp	1.0	76.0
psbC_p4	GTTTCCCAAGAAATCCGTGC	CCGAGCCAGAACAGAAAAGA	50.0	50.0	57.7	57.8	1583 bp	1.0	75.9
psbC_p5	TTCCCAAGAAATCCGTGCAG	CCGAGCCAGAACAGAAAAGA	50.0	50.0	58.5	57.8	1581 bp	1.0	74.9