Hotspot

petA

LSC · 105016-105978 · 963 bp

Back to Alcantarea

Pi 0.00208

MarkerSeek score 54.80

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	CAGACTCGTGCATAGGGAAC	AAACAAAGCAAGCAAAAGAA	55.0	30.0	58.1	52.9	1141 bp	1.0	40.1
petA_p2	CAGACTCGTGCATAGGGAAC	AGCAAGCAAAAGAATTTACAGA	55.0	31.8	58.1	54.8	1135 bp	1.0	40.1
petA_p3	CAGACTCGTGCATAGGGAAC	ACAAAGCAAGCAAAAGAATTTACA	55.0	29.2	58.1	56.8	1139 bp	1.0	40.1
petA_p4	CAGACTCGTGCATAGGGAAC	ACAAAGCAAGCAAAAGAATTTACAG	55.0	32.0	58.1	57.8	1139 bp	1.0	40.1
petA_p5	CAGACTCGTGCATAGGGAAC	ACAAAGCAAGCAAAAGAATT	55.0	30.0	58.1	52.7	1139 bp	1.0	40.1