Hotspot

rbcL-accD

LSC · 99107-99845 · 739 bp

Back to Alcantarea

Pi 0.00277

MarkerSeek score 62.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 2
pi: 0.00277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	ATCACTTCCACTGGTATGGC	55.0	50.0	58.0	57.3	956-957 bp	1.0	75.5
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	ATCACTTCCACTGGTATGGC	50.0	50.0	58.2	57.3	961-962 bp	1.0	73.8
rbcL-accD_p3	TCGACTTCGAACCGGTAGAT	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	899-900 bp	1.0	73.8
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	964-965 bp	1.0	73.7
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	ATCACTTCCACTGGTATGGC	50.0	50.0	58.3	57.3	965-966 bp	1.0	73.7