Hotspot

rpl32-trnL-UAG

SSC · 134449-134923 · 475 bp

Back to Albizia

Pi 0.0681

MarkerSeek score 81.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 475
variable_sites: 44
pi: 0.0681
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTCTTTCTACGGGCAACTCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.3	58.0	563 bp	0.333333	47.3
rpl32-trnL-UAG_p2	CTCTTTCTACGGGCAACTCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.3	58.0	564 bp	0.333333	47.3
rpl32-trnL-UAG_p3	CTCTTTCTACGGGCAACTCA	GCTGCATAGCCCAATACCAT	50.0	50.0	57.3	58.1	1152 bp	0.333333	46.7
rpl32-trnL-UAG_p4	CTCTTTCTACGGGCAACTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.3	57.9	573 bp	0.333333	46.4
rpl32-trnL-UAG_p5	CTCTTTCTACGGGCAACTCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.3	57.8	571 bp	0.333333	45.8