Hotspot

rps3-rps19

LSC · 90564-91071 · 508 bp

Back to Albizia

Pi 0.0443

MarkerSeek score 81.80

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 29
pi: 0.0443
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	ATAAACTTCCGGGGACATGC	50.0	50.0	57.5	57.9	537-596 bp	1.0	84.1
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	ATAAACTTCCGGGGACATGC	50.0	50.0	58.6	57.9	531-590 bp	1.0	83.3
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	ATAAACTTCCGGGGACATGC	45.0	50.0	57.0	57.9	575-634 bp	1.0	79.8
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	ATAAACTTCCGGGGACATGC	45.0	50.0	57.0	57.9	530-589 bp	1.0	79.5
rps3-rps19_p5	GTCTGAAACCGAGCGGATT	ATAAACTTCCGGGGACATGC	52.6	50.0	57.9	57.9	531-590 bp	1.0	78.7