Hotspot

ndhC-trnV-UAC

LSC · 55897-56566 · 670 bp

Back to Albizia

Pi 0.0258

MarkerSeek score 72.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 25
pi: 0.0258
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGCCCTTTATTAATCGGGG	AAAAGATGCACGTTGGGTCT	50.0	45.0	57.3	58.0	888-1241 bp	1.0	78.0
ndhC-trnV-UAC_p2	TGGCCCTTTATTAATCGGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.3	57.8	817-1170 bp	1.0	76.8
ndhC-trnV-UAC_p3	TGGCCCTTTATTAATCGGGG	GATGCACGTTGGGTCTGTAA	50.0	50.0	57.3	58.2	884-1237 bp	1.0	76.6
ndhC-trnV-UAC_p4	TGGCCCTTTATTAATCGGGG	ATGCACGTTGGGTCTGTAAA	50.0	45.0	57.3	57.7	883-1236 bp	1.0	76.1
ndhC-trnV-UAC_p5	TGGCCCTTTATTAATCGGGG	ACGTTGGGTCTGTAAAGCAA	50.0	45.0	57.3	57.7	879-1232 bp	1.0	75.5