Hotspot

trnF-GAA-ndhJ

LSC · 53423-54175 · 753 bp

Back to Albizia

Pi 0.0109

MarkerSeek score 67.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 11
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	863-894 bp	1.0	83.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	827-858 bp	1.0	83.6
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	864-895 bp	1.0	83.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	828-859 bp	1.0	83.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATGGCCCTTGCGTAAAGATT	55.0	45.0	58.0	57.9	853-884 bp	1.0	82.8