Hotspot

atpF-atpH

LSC · 15043-15806 · 764 bp

Back to Albizia

Pi 0.0283

MarkerSeek score 66.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 29
pi: 0.0283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TAGAAGGGATCGCGAGACAA	50.0	50.0	57.9	58.2	929-973 bp	1.0	83.6
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCAGGTCAAGCTGTAGAAGG	50.0	55.0	57.9	58.3	942-986 bp	1.0	83.4
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CCGTCGGACTTGCTTCTATT	50.0	50.0	57.9	57.7	989-1033 bp	1.0	83.0
atpF-atpH_p4	GGGATCATGCACCTTTTCCT	TAGAAGGGATCGCGAGACAA	50.0	50.0	57.9	58.2	1090-1134 bp	1.0	82.6
atpF-atpH_p5	GGGATCATGCACCTTTTCCT	GCAGGTCAAGCTGTAGAAGG	50.0	55.0	57.9	58.3	1103-1147 bp	1.0	82.3