Hotspot

trnG-UCC-trnR-UCU

LSC · 11207-11569 · 363 bp

Back to Albizia

Pi 0.0349

MarkerSeek score 67.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 363
variable_sites: 19
pi: 0.0349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	443-459 bp	1.0	66.7
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	446-462 bp	1.0	66.5
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	449-465 bp	1.0	66.0
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	452-468 bp	1.0	65.8
trnG-UCC-trnR-UCU_p5	ACCAAAGTTTCACGTTCGGT	AGAAGACCTCTGTCCTATCCA	45.0	47.6	58.3	56.9	513-529 bp	1.0	64.4