Hotspot

trnS-GCU-trnG-UCC

LSC · 9120-10415 · 1296 bp

Back to Albizia

Pi 0.0325

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 45
pi: 0.0325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1064-1376 bp	1.0	78.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1063-1375 bp	1.0	78.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.2	58.0	1118-1430 bp	1.0	77.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	1053-1365 bp	1.0	77.2
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	1052-1364 bp	1.0	77.2