Hotspot

thiG

Genome · 35005-35823 · 819 bp

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Pi 0.0171
MarkerSeek score 56.10
Primer available yes
Label thiG

Feature Metadata

label_name
thiG
feature_type
gene
region
Genome
length_bp
819
variable_sites
30
pi
0.0171
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
thiG_p1 TGTTGTACTCGTTCACGCAA TTTGCGAGTGTAGCTCAGTG 45.0 50.0 58.1 58.2 1342-1355 bp 1.0 81.0
thiG_p2 ATGTTGTACTCGTTCACGCA TTTGCGAGTGTAGCTCAGTG 45.0 50.0 57.9 58.2 1343-1356 bp 1.0 80.5
thiG_p3 GGGGCAATAAAGCTTCCCAT TTTGCGAGTGTAGCTCAGTG 50.0 50.0 58.2 58.2 1549-1562 bp 0.8 70.9
thiG_p4 CGTGATATCACCTGTTGGGG TTTGCGAGTGTAGCTCAGTG 55.0 50.0 58.0 58.2 1566 bp 0.2 45.9
thiG_p5 CGTGATATCACCTGTTGGGG ACGAAGGATGAACCACAAGC 55.0 50.0 58.0 58.5 1971 bp 0.2 43.8