Hotspot

petF

Genome · 107954-108247 · 294 bp

Pi 0.0595

MarkerSeek score 72.70

Primer available yes

Label petF

Feature Metadata

label_name: petF
feature_type: gene
region: Genome
length_bp: 294
variable_sites: 34
pi: 0.0595
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petF_p1	ACACGGTACCATGGTAATGC	AAGCAGCTGCAGAAGAAACT	50.0	45.0	58.0	58.0	682-688 bp	1.0	86.4
petF_p2	TGACGCCACATTGGGTTTAA	AAGCAGCTGCAGAAGAAACT	45.0	45.0	58.0	58.0	832-838 bp	1.0	85.4
petF_p3	TTGACGCCACATTGGGTTTA	AAGCAGCTGCAGAAGAAACT	45.0	45.0	58.0	58.0	833-839 bp	1.0	85.4
petF_p4	TGCCAAACAGCAGCTAAGAA	AAGCAGCTGCAGAAGAAACT	45.0	45.0	58.0	58.0	1003-1009 bp	1.0	84.4
petF_p5	GGTTGGACTTGGCCTGTTAA	AAGCAGCTGCAGAAGAAACT	50.0	45.0	58.0	58.0	1201-1207 bp	1.0	83.9