Hotspot

trnQ-UUG-ycf65

Genome · 128568-129117 · 550 bp

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Pi 0.0553
MarkerSeek score 78.60
Primer available yes
Label trnQ-UUG-ycf65

Feature Metadata

label_name
trnQ-UUG-ycf65
feature_type
igs
region
Genome
length_bp
550
variable_sites
65
pi
0.0553
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-ycf65_p1 CTTTGACTCCGCCATTCGTA GCTAATGGGGTTCAGCCTAC 50.0 55.0 58.0 58.0 1012-1021 bp 1.0 85.8
trnQ-UUG-ycf65_p2 TTTGACTCCGCCATTCGTAG GCTAATGGGGTTCAGCCTAC 50.0 55.0 58.0 58.0 1011-1020 bp 1.0 85.8
trnQ-UUG-ycf65_p3 CGAATCCCTCCTAGCTCAGA GCTAATGGGGTTCAGCCTAC 55.0 55.0 58.0 58.0 1101-1110 bp 1.0 85.4
trnQ-UUG-ycf65_p4 AATTTGTCTTGCAGCAGGGA GCTAATGGGGTTCAGCCTAC 45.0 55.0 58.0 58.0 1616-1634 bp 0.4 58.3
trnQ-UUG-ycf65_p5 TTTGTCTTGCAGCAGGGATT GCTAATGGGGTTCAGCCTAC 45.0 55.0 58.0 58.0 1614-1632 bp 0.4 58.3