Hotspot

ilvB-rps4

Genome · 127552-127630 · 79 bp

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Pi 0.0835
MarkerSeek score 73.10
Primer available yes
Label ilvB-rps4

Feature Metadata

label_name
ilvB-rps4
feature_type
igs
region
Genome
length_bp
79
variable_sites
13
pi
0.0835
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ilvB-rps4_p1 TGGCTTACCTGCTGCTATTG AATCCCTGCTGCAAGACAAA 50.0 45.0 58.0 58.0 888-894 bp 1.0 83.3
ilvB-rps4_p2 TGGCTTACCTGCTGCTATTG TCCCTGCTGCAAGACAAATT 50.0 45.0 58.0 58.0 886-892 bp 1.0 83.3
ilvB-rps4_p3 GCTGGGTTAGGTACAATGGG TCCCTGCTGCAAGACAAATT 55.0 45.0 57.9 58.0 909-915 bp 1.0 83.3
ilvB-rps4_p4 CTGGGTTAGGTACAATGGGC AATCCCTGCTGCAAGACAAA 55.0 45.0 57.9 58.0 910 bp 0.2 47.6
ilvB-rps4_p5 CTGGGTTAGGTACAATGGGC TCCCTGCTGCAAGACAAATT 55.0 45.0 57.9 58.0 908 bp 0.2 47.6