Hotspot

psbC-ycf41

Genome · 123363-123506 · 144 bp

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Pi 0.0797
MarkerSeek score 77.80
Primer available yes
Label psbC-ycf41

Feature Metadata

label_name
psbC-ycf41
feature_type
igs
region
Genome
length_bp
144
variable_sites
25
pi
0.0797
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-ycf41_p1 GCTGCTGCAGGTTTTGAAAA AACGAGGTAAAAATTATTGTCA 45.0 27.3 58.1 52.1 266-271 bp 1.0 48.1
psbC-ycf41_p2 GCTGCTGCAGGTTTTGAAAA ACGAGGTAAAAATTATTGTCAA 45.0 27.3 58.1 52.1 265-270 bp 1.0 48.1
psbC-ycf41_p3 AGTTGGTCATTGGTGGCATT AACGAGGTAAAAATTATTGTCA 45.0 27.3 58.0 52.1 303-308 bp 1.0 47.1
psbC-ycf41_p4 AGTTGGTCATTGGTGGCATT ACGAGGTAAAAATTATTGTCAA 45.0 27.3 58.0 52.1 302-307 bp 1.0 47.1
psbC-ycf41_p5 AGTTGGTCATTGGTGGCATT ACGAGGTAAAAATTATTGTCAAA 45.0 26.1 58.0 52.9 302-307 bp 1.0 47.1