Hotspot

trnL-UAG-rns

Genome · 82142-82568 · 427 bp

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Pi 0.0403
MarkerSeek score 77.00
Primer available yes
Label trnL-UAG-rns

Feature Metadata

label_name
trnL-UAG-rns
feature_type
igs
region
Genome
length_bp
427
variable_sites
38
pi
0.0403
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rns_p1 AATACGAACGTGGCGGAATT GCATGTGTAAAGCATACCGC 45.0 50.0 58.3 57.9 563-579 bp 1.0 82.4
trnL-UAG-rns_p2 AATACGAACGTGGCGGAATT TTCGTACGACTTGCATGTGT 45.0 45.0 58.3 57.9 575-591 bp 1.0 81.9
trnL-UAG-rns_p3 GTTCAAGTCTCTCCGTTCGT GCATGTGTAAAGCATACCGC 50.0 50.0 57.6 57.9 499-515 bp 1.0 81.9
trnL-UAG-rns_p4 AATACGAACGTGGCGGAATT ACACTTTCGTACGACTTGCA 45.0 45.0 58.3 57.8 580-596 bp 1.0 81.3
trnL-UAG-rns_p5 AATACGAACGTGGCGGAATT TGTGTAAAGCATACCGCCAG 45.0 50.0 58.3 58.3 560-576 bp 1.0 81.1