Hotspot

psbA-cbbx

Genome · 74679-75443 · 765 bp

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Pi 0.0507
MarkerSeek score 84.30
Primer available yes
Label psbA-cbbx

Feature Metadata

label_name
psbA-cbbx
feature_type
igs
region
Genome
length_bp
765
variable_sites
84
pi
0.0507
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-cbbx_p1 CTGCTCCTTCTGTTGTTGGT GAGCTCGAATGAGACAAGCA 50.0 50.0 58.0 58.0 896-924 bp 1.0 90.2
psbA-cbbx_p2 TGTTGCGATTTCTGCTCCTT GAGCTCGAATGAGACAAGCA 45.0 50.0 58.1 58.0 907-935 bp 1.0 89.4
psbA-cbbx_p3 TTCCTGTTGCGATTTCTGCT GAGCTCGAATGAGACAAGCA 45.0 50.0 58.1 58.0 911-939 bp 1.0 89.4
psbA-cbbx_p4 CTGCTCCTTCTGTTGTTGGT TAAAACGTCGTGAACAGCCA 50.0 45.0 58.0 58.1 956-984 bp 1.0 89.1
psbA-cbbx_p5 TGGTGTTTTCGGTGGTTCAT GAGCTCGAATGAGACAAGCA 45.0 50.0 57.9 58.0 1348-1376 bp 1.0 86.5