Hotspot

psbK-ccsA

Genome · 57873-58188 · 316 bp

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Pi 0.0278
MarkerSeek score 68.80
Primer available yes
Label psbK-ccsA

Feature Metadata

label_name
psbK-ccsA
feature_type
igs
region
Genome
length_bp
316
variable_sites
19
pi
0.0278
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-ccsA_p1 TGAAGCTTGCCAAAGGAAGG GGATTACATAGCTACGGTTGGT 50.0 45.5 58.7 57.7 460-466 bp 1.0 60.3
psbK-ccsA_p2 TGAAGCTTGCCAAAGGAAGG ACATAGCTACGGTTGGTTTACA 50.0 40.9 58.7 57.5 455-461 bp 1.0 58.7
psbK-ccsA_p3 TGAAGCTTGCCAAAGGAAGG AGGATTACATAGCTACGGTTGG 50.0 45.5 58.7 57.4 461-467 bp 1.0 58.1
psbK-ccsA_p4 TGAAGCTTGCCAAAGGAAGG AAGGATTACATAGCTACGGTTGG 50.0 43.5 58.7 58.0 462-468 bp 1.0 54.6
psbK-ccsA_p5 TGAAGCTTGCCAAAGGAAGG GGATTACATAGCTACGGTTGG 50.0 47.6 58.7 55.9 460-466 bp 1.0 54.5