Hotspot

trnL-UAA-trnC-GCA

Genome · 49204-49281 · 78 bp

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Pi 0.0628
MarkerSeek score 80.20
Primer available yes
Label trnL-UAA-trnC-GCA

Feature Metadata

label_name
trnL-UAA-trnC-GCA
feature_type
igs
region
Genome
length_bp
78
variable_sites
10
pi
0.0628
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnC-GCA_p1 TTAAGTCCGTTGCGTCTACC CCAGTTCGAATCTGGGTGTC 50.0 55.0 57.6 58.3 138 bp 1.0 80.8
trnL-UAA-trnC-GCA_p2 TTAAGTCCGTTGCGTCTACC TAAGGCCGTGGATTGCAAAT 50.0 45.0 57.6 58.2 167 bp 1.0 80.7
trnL-UAA-trnC-GCA_p3 TTAAGTCCGTTGCGTCTACC CCGTGGATTGCAAATCCTCT 50.0 50.0 57.6 58.2 162 bp 1.0 80.2
trnL-UAA-trnC-GCA_p4 TAAGTCCGTTGCGTCTACCA TAAGGCCGTGGATTGCAAAT 50.0 45.0 58.8 58.2 166 bp 1.0 77.6
trnL-UAA-trnC-GCA_p5 TAAGTCCGTTGCGTCTACCA CCGTGGATTGCAAATCCTCT 50.0 50.0 58.8 58.2 161 bp 1.0 77.0