Hotspot

ilvH-ycf34

Genome · 48301-48570 · 270 bp

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Pi 0.0395
MarkerSeek score 73.20
Primer available yes
Label ilvH-ycf34

Feature Metadata

label_name
ilvH-ycf34
feature_type
igs
region
Genome
length_bp
270
variable_sites
25
pi
0.0395
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ilvH-ycf34_p1 GAGTTCGAAGCTGATACGGG TGCAAATCGTGAGGGTTCAA 55.0 45.0 58.2 58.0 701-721 bp 1.0 85.9
ilvH-ycf34_p2 AGCAGACCCTGGGAAAATTG TGCAAATCGTGAGGGTTCAA 50.0 45.0 58.1 58.0 834-854 bp 1.0 85.8
ilvH-ycf34_p3 CAGCAGACCCTGGGAAAATT TGCAAATCGTGAGGGTTCAA 50.0 45.0 58.1 58.0 835-855 bp 1.0 85.8
ilvH-ycf34_p4 AAGGATGCAGGAGGGTTAGT TGCAAATCGTGAGGGTTCAA 50.0 45.0 58.0 58.0 1187-1207 bp 1.0 85.3
ilvH-ycf34_p5 ATGCAGGAGGGTTAGTACGT TGCAAATCGTGAGGGTTCAA 50.0 45.0 58.1 58.0 1183-1203 bp 1.0 84.4