Hotspot

rpl16

LSC · 83140-84575 · 1436 bp

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Pi 0.00344

MarkerSeek score 49.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1436
variable_sites: 13
pi: 0.00344
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTTCTTTCAATGCAAAGGACG	ACTCGTCCATTCTTTTTCTGT	40.9	38.1	58.0	55.5	1652-1654 bp	1.0	40.8
rpl16_p2	TGTTCTTTCAATGCAAAGGACG	CTCGTCCATTCTTTTTCTGTTCA	40.9	39.1	58.0	57.3	1651-1653 bp	1.0	40.8
rpl16_p3	TGTTCTTTCAATGCAAAGGACG	ACTCGTCCATTCTTTTTCTGTTC	40.9	39.1	58.0	57.3	1652-1654 bp	1.0	40.8
rpl16_p4	GTTCTTTCAATGCAAAGGACGA	ACTCGTCCATTCTTTTTCTGT	40.9	38.1	57.8	55.5	1651-1653 bp	1.0	40.8
rpl16_p5	TGTTCTTTCAATGCAAAGGACG	TCGTCCATTCTTTTTCTGTTCA	40.9	36.4	58.0	56.2	1650-1652 bp	1.0	40.8