Hotspot

ndhD

SSC · 118042-119571 · 1530 bp

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Pi 0.00248
MarkerSeek score 47.00
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1530
variable_sites
10
pi
0.00248
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GCTAATGAGGCATGGGGATC TCTGCTCCAAGAACAGAGGA 55.0 50.0 58.2 58.0 2242-2244 bp 1.0 79.3
ndhD_p2 GCTAATGAGGCATGGGGATC AAGATGTGAATCCGCCTGTC 55.0 50.0 58.2 58.0 2207-2209 bp 1.0 79.2
ndhD_p3 GGGAGCAGTATGGGCTAATG TCTGCTCCAAGAACAGAGGA 55.0 50.0 57.8 58.0 2255-2257 bp 1.0 79.2
ndhD_p4 GCTAATGAGGCATGGGGATC ACCGACAAAACTCGTACTCG 55.0 50.0 58.2 58.0 2074-2075 bp 1.0 79.2
ndhD_p5 GCTAATGAGGCATGGGGATC AGCGTATTTGCCAGGTAAGG 55.0 50.0 58.2 58.0 2563-2565 bp 1.0 79.1