Hotspot

ycf1-ndhF

SSC · 112115-112137 · 23 bp

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Pi 0.0725

MarkerSeek score 58.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 23
variable_sites: 5
pi: 0.0725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGAGTAAACAGGA	GGAAACATGACACGGTCCTT	50.0	50.0	58.0	58.1	949-956 bp	0.666667	65.6
ycf1-ndhF_p2	GCAGAATACCGTCACCCATT	GGAAACATGACACGGTCCTT	50.0	50.0	58.0	58.1	1059-1066 bp	0.666667	65.5
ycf1-ndhF_p3	AGAAACAGCTTCCGAAACGA	GGAAACATGACACGGTCCTT	45.0	50.0	57.8	58.1	965-972 bp	0.666667	63.9
ycf1-ndhF_p4	GGAACAAGAGGAATCCACCG	GGAAACATGACACGGTCCTT	55.0	50.0	58.3	58.1	932-939 bp	0.666667	63.7
ycf1-ndhF_p5	TCCGAAACGAAGGGGAGTAA	GGAAACATGACACGGTCCTT	50.0	50.0	58.4	58.1	955-962 bp	0.666667	62.9