Hotspot

petA-psbJ

LSC · 64814-65843 · 1030 bp

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Pi 0.00861

MarkerSeek score 51.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 26
pi: 0.00861
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TACGTGTCCAAGGGCTTTTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1168-1169 bp	1.0	78.0
petA-psbJ_p2	CATTACGTGTCCAAGGGCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1171-1172 bp	1.0	77.5
petA-psbJ_p3	TTACGTGTCCAAGGGCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1169-1170 bp	1.0	76.1
petA-psbJ_p4	ATTACGTGTCCAAGGGCTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	1170-1171 bp	1.0	74.3
petA-psbJ_p5	CGTGTCCAAGGGCTTTTCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1166-1167 bp	1.0	73.5