Hotspot

rps4-trnT-UGU

LSC · 47364-47733 · 370 bp

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Pi 0.00559

MarkerSeek score 49.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 5
pi: 0.00559
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	524-533 bp	1.0	78.9
rps4-trnT-UGU_p2	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	492-501 bp	1.0	78.2
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.3	57.5	532-541 bp	1.0	76.0
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	CAGAGGTTAGAGCATCGCAT	55.0	50.0	58.3	57.5	522-531 bp	1.0	75.8
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	TCAGAGGTTAGAGCATCGCA	55.0	50.0	58.3	58.5	523-532 bp	1.0	75.8