Hotspot

ycf3-trnS-GGA

LSC · 45546-46367 · 822 bp

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Pi 0.00454
MarkerSeek score 51.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
822
variable_sites
10
pi
0.00454
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA GGTGAATTAGGTGACGGTCC 45.0 55.0 56.4 58.0 1024-1033 bp 1.0 67.9
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA GGTGAATTAGGTGACGGTCC 45.0 55.0 56.4 58.0 1025-1034 bp 1.0 67.9
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 985-994 bp 1.0 67.4
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 986-995 bp 1.0 67.4
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTGAA 45.0 45.0 56.4 57.8 956-965 bp 1.0 66.6