Hotspot

trnK-UUU-rps16

LSC · 4297-5045 · 749 bp

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Pi 0.00695
MarkerSeek score 54.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
749
variable_sites
12
pi
0.00695
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTCGTGGTCTTCCAAACCTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.0 58.2 958-959 bp 1.0 79.0
trnK-UUU-rps16_p2 GTCGTGGTCTTCCAAACCTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.0 58.2 957-958 bp 1.0 79.0
trnK-UUU-rps16_p3 CGAATTCCTCGCTTCATCCA CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.1 58.2 924-925 bp 1.0 78.8
trnK-UUU-rps16_p4 CGAATTCCTCGCTTCATCCA CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.1 58.2 923-924 bp 1.0 78.8
trnK-UUU-rps16_p5 GTCGTGGTCTTCCAAACCTT ACTTCCTTGAAAAAGGCGCT 50.0 45.0 58.0 58.3 962-963 bp 1.0 78.3