Hotspot

ccsA

SSC · 117641-118612 · 972 bp

Back to Akebia

Pi 0.00206
MarkerSeek score 33.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
4
pi
0.00206
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 CCCTCCTCTTTGTGCGTTTA TGTATTTGTCAGGTCCTTTCC 50.0 42.9 57.8 55.5 1025 bp 1.0 50.9
ccsA_p2 CCCTCCTCTTTGTGCGTTTA TTGTATTTGTCAGGTCCTTTCC 50.0 40.9 57.8 56.3 1026 bp 1.0 49.0
ccsA_p3 ACATTGTTGAAGGACCCTCC TGTATTTGTCAGGTCCTTTCC 50.0 42.9 57.4 55.5 1039 bp 1.0 47.6
ccsA_p4 ACATTGTTGAAGGACCCTCC TTGTATTTGTCAGGTCCTTTCC 50.0 40.9 57.4 56.3 1040 bp 1.0 45.7
ccsA_p5 CCCTCCTCTTTGTGCGTTTA ATTGTATTTGTCAGGTCCTTTCC 50.0 39.1 57.8 56.8 1027 bp 1.0 45.0