Hotspot

trnV-GAC-rps12

IRa · 141811-143715 · 1905 bp

Back to Akebia

Pi 0.000525
MarkerSeek score 50.60
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1905
variable_sites
2
pi
0.000525
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 2043-2062 bp 1.0 78.1
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1982-2001 bp 1.0 77.8
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1986-2005 bp 1.0 77.7
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1981-2000 bp 1.0 77.7
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1984-2003 bp 1.0 76.8