Hotspot

ndhF-rpl32

SSC · 115221-116293 · 1073 bp

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Pi 0.00606
MarkerSeek score 66.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1073
variable_sites
13
pi
0.00606
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AACCGGAAGTGGAACGAAAG AAAGCTTTTACCGCTGTCCA 50.0 45.0 58.4 58.0 1194-1226 bp 1.0 79.4
ndhF-rpl32_p2 AACCGGAAGTGGAACGAAAG AAGCTTTTACCGCTGTCCAA 50.0 45.0 58.4 58.0 1193-1225 bp 1.0 79.4
ndhF-rpl32_p3 ATTCTTTGTTGCCGTCGGAA AAAGCTTTTACCGCTGTCCA 45.0 45.0 58.4 58.0 1245-1277 bp 1.0 79.3
ndhF-rpl32_p4 ATTCTTTGTTGCCGTCGGAA AAGCTTTTACCGCTGTCCAA 45.0 45.0 58.4 58.0 1244-1276 bp 1.0 79.3
ndhF-rpl32_p5 GAACCGGAAGTGGAACGAAA AAGCTTTTACCGCTGTCCAA 50.0 45.0 58.4 58.0 1194-1226 bp 1.0 79.3