Hotspot

petA-psbJ

LSC · 65179-66189 · 1011 bp

Back to Akebia

Pi 0.0106
MarkerSeek score 55.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1011
variable_sites
21
pi
0.0106
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CAGGCCCAGAACTTCTTGTT ACACAATCAAACCCGAACGA 50.0 45.0 58.0 58.0 1588-1616 bp 1.0 82.6
petA-psbJ_p2 CAGGCCCAGAACTTCTTGTT GGACTAGCTGTACCTACCGT 50.0 55.0 58.0 58.0 1681-1709 bp 1.0 82.4
petA-psbJ_p3 CAGGCCCAGAACTTCTTGTT GAGCCGACAAGGAATTCCAT 50.0 50.0 58.0 58.0 1822-1850 bp 1.0 82.2
petA-psbJ_p4 AATGGTAAAAAGGGGGCGTT ACACAATCAAACCCGAACGA 45.0 45.0 58.0 58.0 2027-2055 bp 1.0 81.9
petA-psbJ_p5 AATGGTAAAAAGGGGGCGTT GAGCCGACAAGGAATTCCAT 45.0 50.0 58.0 58.0 2261-2289 bp 1.0 81.6