Hotspot

accD-psaI

LSC · 60621-61296 · 676 bp

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Pi 0.0074
MarkerSeek score 39.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
676
variable_sites
10
pi
0.0074
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CACGGTTTCTTTCCCTTGAA GCCATTGAAATTGCCGGAAA 45.0 45.0 56.2 57.9 786-797 bp 1.0 66.6
accD-psaI_p2 CACGGTTTCTTTCCCTTGAA GAAGCCATTGAAATTGCCGG 45.0 50.0 56.2 58.4 789-800 bp 1.0 64.5
accD-psaI_p3 CACGGTTTCTTTCCCTTGAA GCCGGAAATACTAGGCCTAC 45.0 55.0 56.2 57.5 774-785 bp 1.0 63.7
accD-psaI_p4 CACGGTTTCTTTCCCTTGAAT GCCATTGAAATTGCCGGAAA 42.9 45.0 56.7 57.9 786-797 bp 1.0 62.9
accD-psaI_p5 CACGGTTTCTTTCCCTTGAA AGAAGCCATTGAAATTGCCG 45.0 45.0 56.2 57.3 790-801 bp 1.0 62.0