Hotspot

trnS-UGA-psbZ

LSC · 37539-37879 · 341 bp

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Pi 0.00293
MarkerSeek score 36.60
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
341
variable_sites
2
pi
0.00293
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAAGACCGGAGCTATCAACC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 477 bp 1.0 72.2
trnS-UGA-psbZ_p2 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 410 bp 1.0 72.0
trnS-UGA-psbZ_p3 CTATCAACCACTCGGCCATC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 466 bp 1.0 71.7
trnS-UGA-psbZ_p4 CTATCAACCACTCGGCCATC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 399 bp 1.0 71.4
trnS-UGA-psbZ_p5 CGAGCAAAAGGAGAGAGAGG ACCAACCATCAGAAGAAGCA 55.0 45.0 57.7 57.0 542 bp 1.0 70.3