Hotspot

trnC-GCA-petN

LSC · 28383-29241 · 859 bp

Back to Akebia

Pi 0.0146
MarkerSeek score 55.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
859
variable_sites
25
pi
0.0146
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.1 58.0 922-924 bp 1.0 81.5
trnC-GCA-petN_p2 GTATCATTTTGGCGGCATGG TTAAAGCAGCCCAAGCAAGA 50.0 45.0 57.9 58.0 974-976 bp 1.0 80.9
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 50.0 50.0 58.1 57.8 916-918 bp 1.0 80.0
trnC-GCA-petN_p4 GTATCATTTTGGCGGCATGG CAGCCCAAGCAAGACTTACT 50.0 50.0 57.9 57.8 968-970 bp 1.0 79.4
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 50.0 45.0 58.4 58.0 940-942 bp 1.0 79.2