Hotspot

trnS-GCU-trnG-UCC

LSC · 8638-9369 · 732 bp

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Pi 0.0041
MarkerSeek score 45.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
732
variable_sites
6
pi
0.0041
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 806-810 bp 1.0 52.1
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 809-813 bp 1.0 51.0
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 808-812 bp 1.0 51.0
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 807-811 bp 1.0 50.2
trnS-GCU-trnG-UCC_p5 ATTAGCAATCCGCCGCTTTA CAAATTGAAAGACCCCTTAACCA 45.0 39.1 58.0 57.2 846-850 bp 1.0 50.0