Hotspot

psbI-trnS-GCU

LSC · 8372-8549 · 178 bp

Back to Akebia

Pi 0.0253
MarkerSeek score 60.80
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
178
variable_sites
9
pi
0.0253
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 AATCCCGGACGTGAGGAATA TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.2 58.0 266-267 bp 1.0 81.0
psbI-trnS-GCU_p2 ATCCCGGACGTGAGGAATAA TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.2 58.0 265-266 bp 1.0 81.0
psbI-trnS-GCU_p3 TAATCCCGGACGTGAGGAAT TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.2 58.0 267-268 bp 1.0 81.0
psbI-trnS-GCU_p4 AATCCCGGACGTGAGGAATA ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.2 58.1 290-291 bp 1.0 80.2
psbI-trnS-GCU_p5 ATCCCGGACGTGAGGAATAA ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.2 58.1 289-290 bp 1.0 80.2