Hotspot

ndhF

SSC · 107589-109829 · 2241 bp

Pi 0.00756

MarkerSeek score 59.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 84
pi: 0.00756
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCATTTCTGAAGAGTCT	AAAGAGGTAAGGTCCGGTGA	42.9	50.0	56.0	58.0	2361-2372 bp	1.0	59.1
ndhF_p2	AGACCCATTTCTGAAGAGTCT	AAGAGGTAAGGTCCGGTGAA	42.9	50.0	56.0	58.0	2360-2371 bp	1.0	59.1
ndhF_p3	GACAGGCTATCAAAATAGACCCA	AAAGAGGTAAGGTCCGGTGA	43.5	50.0	58.0	58.0	2377-2388 bp	1.0	58.9
ndhF_p4	GACAGGCTATCAAAATAGACCCA	AAGAGGTAAGGTCCGGTGAA	43.5	50.0	58.0	58.0	2376-2387 bp	1.0	58.9
ndhF_p5	ACAGGCTATCAAAATAGACCCA	AAGAGGTAAGGTCCGGTGAA	40.9	50.0	56.9	58.0	2375-2386 bp	1.0	58.1