Hotspot

ccsA

SSC · 111115-112086 · 972 bp

Back to Ajuga

Pi 0.00558
MarkerSeek score 50.50
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
31
pi
0.00558
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TAGACACGCTGCTCTTAGGA TCAGTAGCTTTTCGATGGCC 50.0 50.0 57.9 58.0 1637-1768 bp 1.0 86.1
ccsA_p2 TAGACACGCTGCTCTTAGGA CAGTAGCTTTTCGATGGCCT 50.0 50.0 57.9 58.0 1636-1767 bp 1.0 86.1
ccsA_p3 AAATTGGTAGACACGCTGCT TCAGTAGCTTTTCGATGGCC 45.0 50.0 57.8 58.0 1644-1775 bp 1.0 85.6
ccsA_p4 AAATTGGTAGACACGCTGCT CAGTAGCTTTTCGATGGCCT 45.0 50.0 57.8 58.0 1643-1774 bp 1.0 85.6
ccsA_p5 GTAGACACGCTGCTCTTAGG TCAGTAGCTTTTCGATGGCC 55.0 50.0 57.8 58.0 1638-1769 bp 1.0 85.6