Hotspot

ndhE-ndhG

SSC · 114772-115004 · 233 bp

Back to Ajuga

Pi 0.0241
MarkerSeek score 63.80
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
233
variable_sites
33
pi
0.0241
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 GACACATAAGGGCTCTAACCA CGCCTTAATAGGTGCTATTGC 47.6 47.6 57.1 57.2 355-377 bp 1.0 61.4
ndhE-ndhG_p2 AGTTCAAGACACATAAGGGCTC CGCCTTAATAGGTGCTATTGC 45.5 47.6 58.1 57.2 362-384 bp 1.0 60.1
ndhE-ndhG_p3 GTTCAAGACACATAAGGGCTCT CGCCTTAATAGGTGCTATTGC 45.5 47.6 58.1 57.2 361-383 bp 1.0 60.1
ndhE-ndhG_p4 AGTTCAAGACACATAAGGGCT CGCCTTAATAGGTGCTATTGC 42.9 47.6 56.9 57.2 362-384 bp 1.0 59.9
ndhE-ndhG_p5 TCAAGACACATAAGGGCTCT CGCCTTAATAGGTGCTATTGC 45.0 47.6 55.9 57.2 359-381 bp 1.0 59.4