Hotspot

pafII-cemA

LSC · 59064-59715 · 652 bp

Back to Ajuga

Pi 0.00725
MarkerSeek score 63.70
Primer available yes
Label pafII-cemA

Feature Metadata

label_name
pafII-cemA
feature_type
igs
region
LSC
length_bp
652
variable_sites
26
pi
0.00725
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafII-cemA_p1 CGTTGACCCGTACTGATGAG GAAATCCACCAGGGCAGAAA 55.0 50.0 58.1 58.1 802-959 bp 1.0 83.6
pafII-cemA_p2 GAACAAAAAGCCGCGGAATT GAAATCCACCAGGGCAGAAA 45.0 50.0 58.2 58.1 761-918 bp 1.0 82.7
pafII-cemA_p3 CGTTGACCCGTACTGATGAG TCCACCAGGGCAGAAATACT 55.0 50.0 58.1 58.3 798-955 bp 1.0 81.6
pafII-cemA_p4 TTGGCCTATTTCTTGCGTGT GAAATCCACCAGGGCAGAAA 45.0 50.0 58.1 58.1 762 bp 0.066667 41.3
pafII-cemA_p5 TTGGCCTATTTCTTGCGTGT TCCACCAGGGCAGAAATACT 45.0 50.0 58.1 58.3 758 bp 0.066667 39.3