Hotspot

ndhC-trnV-UAC

LSC · 49261-50094 · 834 bp

Back to Ajuga

Pi 0.0142
MarkerSeek score 65.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
834
variable_sites
48
pi
0.0142
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.7 58.7 339-887 bp 1.0 48.6
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.7 57.8 351-899 bp 1.0 48.5
ndhC-trnV-UAC_p3 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.7 57.5 350-898 bp 1.0 48.5
ndhC-trnV-UAC_p4 ATCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 352-900 bp 1.0 48.5
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAAAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 351-899 bp 1.0 48.5