Hotspot

petN-psbM

LSC · 28625-29163 · 539 bp

Back to Ajuga

Pi 0.00567
MarkerSeek score 62.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
539
variable_sites
16
pi
0.00567
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 609-666 bp 1.0 77.7
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 605-662 bp 1.0 77.2
petN-psbM_p3 TTTCCCTTTCGCTCGTAGTG TCATTCTAGTTCCTACCGCCT 50.0 47.6 57.9 58.0 621-678 bp 1.0 77.1
petN-psbM_p4 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 608-665 bp 1.0 77.1
petN-psbM_p5 ACATTTTCCCTTTCGCTCGT TCATTCTAGTTCCTACCGCCT 45.0 47.6 58.1 58.0 625-682 bp 1.0 76.9