Hotspot

atpH-atpI

LSC · 13718-14685 · 968 bp

Back to Ajuga

Pi 0.00941
MarkerSeek score 66.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
968
variable_sites
39
pi
0.00941
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA ATCCCCGTCATGTTTCTTGG 45.0 50.0 58.1 57.9 1090-1114 bp 1.0 83.4
atpH-atpI_p2 AACGGAAGCAGCAGAAATCA CCCCGTCATGTTTCTTGGAT 45.0 50.0 58.1 57.9 1088-1112 bp 1.0 83.4
atpH-atpI_p3 AGGTCCAATAGAAGCAAGCC CCCCGTCATGTTTCTTGGAT 50.0 50.0 57.6 57.9 1130-1154 bp 1.0 80.6
atpH-atpI_p4 GTCCAATAGAAGCAAGCCCT ATCCCCGTCATGTTTCTTGG 50.0 50.0 57.6 57.9 1130-1154 bp 1.0 80.6
atpH-atpI_p5 GTCCAATAGAAGCAAGCCCT CCCCGTCATGTTTCTTGGAT 50.0 50.0 57.6 57.9 1128-1152 bp 1.0 80.6