Hotspot

atpA-atpF

LSC · 11761-11874 · 114 bp

Back to Ajuga

Pi 0.0198
MarkerSeek score 63.50
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
114
variable_sites
7
pi
0.0198
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 TTTCGTCAGCTCGAATGGTT ACAAGGAGCTCTAGGGACTC 45.0 55.0 57.8 57.8 211-249 bp 1.0 84.8
atpA-atpF_p2 CCGTCGCCTACTTGAAGTAC ACAAGGAGCTCTAGGGACTC 55.0 55.0 58.1 57.8 299-337 bp 1.0 84.7
atpA-atpF_p3 GTCGCCTACTTGAAGTACGG ACAAGGAGCTCTAGGGACTC 55.0 55.0 58.1 57.8 297-335 bp 1.0 84.7
atpA-atpF_p4 CCGTCGCCTACTTGAAGTAC TTGGGGGCGATGAAAGAAAT 55.0 45.0 58.1 57.8 220-258 bp 0.933333 81.7
atpA-atpF_p5 GTCGCCTACTTGAAGTACGG TTGGGGGCGATGAAAGAAAT 55.0 45.0 58.1 57.8 218-256 bp 0.933333 81.7